abstract：:The polymorphic GC-rich repetitive sequence (PGRS) found on the chromosome of Mycobacterium tuberculosis was characterized by means of mapping, cloning and sequencing. PGRS was present in at least 26 loci and consisted of many tandem repeats of the consensus sequence CGGCGGCAA. As the core of the consensus motif was t...

journal_title：Archives of microbiology

authors： Poulet S,Cole ST

更新日期：1995-02-01 00:00:00

abstract：:Pseudomonas maltophilia CSV89, a bacterium isolated from soil in our laboratory, grows on 1-naphthoic acid as the sole source of carbon and energy. To elucidate the pathway for degradation of 1-naphthoic acid, the metabolites were isolated from spent medium, purified by TLC, and characterized by gas chromatography-mas...

journal_title：Archives of microbiology

authors： Phale PS,Mahajan MC,Vaidyanathan CS

更新日期：1995-01-01 00:00:00

abstract：:The regulation of the syntheses of two arabinan-degrading extracellular enzymes and several intracellular L-arabinose catabolic enzymes was examined in wild-type and carbon catabolite derepressed mutants of Aspergillus nidulans. alpha-L-Arabinofuranosidase B, endoarabinase, L-arabinose reductase, L-arabitol dehydrogen...

journal_title：Archives of microbiology

authors： van der Veen P,Arst HN Jr,Flipphi MJ,Visser J

更新日期：1994-01-01 00:00:00

abstract：:Nodules collected from Alnus nepalensis growing in mixed forest stands at three different sites around Shillong, were crushed in various culture media to obtain isolates of Frankia. The isolates were found to have typical Frankia morphology as revealed by the scanning electron microscope. Seedlings inoculated with iso...

journal_title：Archives of microbiology

authors： Ganesh G,Misra AK,Chapelon C,Normand P

更新日期：1994-01-01 00:00:00

abstract：:We have investigated the endogenous phosphorylation patterns of phosphorylated proteins of Xanthomonas campestris pv. oryzae induced by its bacteriophages. For bacteriophage Xp12-infected cells, at least three phosphoproteins with apparent molecular weights of 28, 28.5 and 45 kDa were detected by in vitro labeling wit...

journal_title：Archives of microbiology

authors： Cheng CM,Tu J,Yang CC,Kuo TT

更新日期：1994-01-01 00:00:00

abstract：:DNA sequence analysis of the structural urease genes from Staphylococcus xylosus revealed that three enzyme subunits are encoded in the order of 11,000, 15,400 and 61,000 (mol. mass), which correspond to the single polypeptide chain of jack bean urease (90,800). Comparing the deduced amino acid sequence of S. xylosus ...

journal_title：Archives of microbiology

authors： Jose J,Schäfer UK,Kaltwasser H

更新日期：1994-01-01 00:00:00

abstract：:Gas chromatography-mass spectrometry studies of the nitrogen isotopic composition of the N-trifluoroacetyl n-butyl ester derivatives of the amino acids from isolated hydrolyzed cyanophycin from 15N-enriched cells led to two major findings: (1) the amino acid composition of this granular polypeptide, isolated using pro...

journal_title：Archives of microbiology

authors： Merritt MV,Sid SS,Mesh L,Allen MM

更新日期：1994-01-01 00:00:00

abstract：:Hyphal development in Candida albicans was blocked by EDTA. This effect was not due to a general growth inhibition since the chelator did not affect protein and DNA synthesis. Recovery of mycelial growth was observed when EDTA-grown cells were incubated at 37 degrees C in EDTA-free medium. High-molecular-weight mannop...

journal_title：Archives of microbiology

authors： Gil ML,Casanova M,Martínez JP

更新日期：1994-01-01 00:00:00

abstract：:The nitrite oxidoreductase (NOR) from the facultative nitrite-oxidizing bacterium Nitrobacter hamburgensis X14 was investigated genetically. In order to develop a probe for the gene norB, the N-terminal amino acid sequence of the NOR beta-subunit (NorB) was determined. Based on that amino acid sequence, an oligonucleo...

journal_title：Archives of microbiology

authors： Kirstein K,Bock E

更新日期：1993-01-01 00:00:00

abstract：:A strain of Pseudomonas fluorescens was capable of co-metabolizing 3-chlorobenzoic acid with the production of a chlorinated catechol black pigment. A peroxidase and another enzymatic activity referred to as a polyphenol oxidase were found to be involved in the oxidation of 4-chlorocatechol to 4-chloro-1,2-benzoquinon...

journal_title：Archives of microbiology

authors： Fava F,Di Gioia D,Romagnoli C,Marchetti L,Mares D

更新日期：1993-01-01 00:00:00

abstract：:Calcium is essential for the growth of rhizobia and the formation of nitrogen-fixing root-nodules on legumes, but its precise role in these processes remains unknown. We have found that Rhizobium fredii USDA208 accumulates a major 38 kDa protein when grown in media supplemented with 0.3-2 microM Cacl2. We have purifie...

journal_title：Archives of microbiology

authors： Krishman HB,Pueppke SG

更新日期：1993-01-01 00:00:00

abstract：:Plasmid-borne hup-lacZ transcriptional fusion constructs were introduced into three separate mutant strains of Bradyrhizobium japonicum which express hydrogenase constitutively (Hupc strains SR470, SR473 and JH101) in both autotrophic and heterotrophic environments. The lacZ structural gene linked directly to the regu...

journal_title：Archives of microbiology

authors： Kim H,Gabel C,Maier RJ

更新日期：1993-01-01 00:00:00

abstract：:An organism from a goldfish aquarium, isolated on barbital medium, was found to be a Gram-positive coccus which divided in alternating planes, often appearing as a doublet or as a tetrad with adjacent sides flattened. It grew well, although slowly, on rich solid medium (LB agar) and in liquid brain-heart infusion at r...

journal_title：Archives of microbiology

authors： Poston JM

更新日期：1993-01-01 00:00:00

abstract：:Purified flagellar filaments isolated from six methanogens were composed of multiple flagellins. Two flagellins were present in Methanococcus deltae (Mr = 34,000 and 32,000), Methanoculleus marisnigri (Mr = 31,000 and 25,500) and Methanococcus jannaschii (Mr = 31,000 and 27,500), three in Methanothermus fervidus (Mr =...

journal_title：Archives of microbiology

authors： Kalmokoff ML,Koval SF,Jarrell KF

更新日期：1992-01-01 00:00:00

abstract：:A bacterium capable of using the carbamate insecticide carbofuran as a sole source of carbon and energy, was isolated from soil. The ability to catabolise carbofuran phenol, produced by cleavage of the carbamate ester linkage of the insecticide, was lost at very high frequency when the bacterium was grown in the absen...

journal_title：Archives of microbiology

authors： Head IM,Cain RB,Suett DL

更新日期：1992-01-01 00:00:00

abstract：:The maleylacetate reductase of 3-chlorobenzoate-grown cells of Pseudomonas sp. strain B13 has been purified 50-fold. The enzyme converted 2-chloromaleylacetate to 3-oxoadipate with temporary occurrence of maleylacetate; 1 mol of chloride was eliminated during the conversion of 1 mol of 2-chloro- and 2,3-dichloromaleyl...

journal_title：Archives of microbiology

authors： Kaschabek SR,Reineke W

更新日期：1992-01-01 00:00:00

abstract：:The major cobamide-containing protein from methanol-utilizing Sporomusa ovata was 8-fold enriched to apparent homogeneity. The protein exhibited a molecular mass of 40 kDa and of 38 kDa determined by gel filtration and by SDS-polyacrylamide gel electrophoresis, respectively. This finding indicates a monomeric protein ...

journal_title：Archives of microbiology

authors： Stupperich E,Aulkemeyer P,Eckerskorn C

更新日期：1992-01-01 00:00:00

abstract：:The thermotolerant methylotroph Bacillus sp. C1 possesses a novel NAD-dependent methanol dehydrogenase (MDH), with distinct structural and mechanistic properties. During growth on methanol and ethanol, MDH was responsible for the oxidation of both these substrates. MDH activity in cells grown on methanol or glucose wa...

journal_title：Archives of microbiology

authors： Arfman N,de Vries KJ,Moezelaar HR,Attwood MM,Robinson GK,van Geel M,Dijkhuizen L

更新日期：1992-01-01 00:00:00

abstract：:A complex polydispersity became apparent when the poly(glycerophosphate) lipoteichoic acid of Enterococcus faecalis was chromatographed on DEAE-Sephadex. The chain length varied between 13 and 33 glycerophosphate residues per lipid anchor. In parallel, the extent of chain glycosylation increased from 0.2 to 0.4 digluc...

journal_title：Archives of microbiology

authors： Leopold K,Fischer W

更新日期：1992-01-01 00:00:00

abstract：:Monoclonal antibodies have been used to determine the presence of cellobiohydrolases I and II (CBH I and II), and endoglucanase I (EG I) on the surface of conidia from Trichoderma reesei QM 9414 and RUT C-30, and 8 other Trichoderma species. For this purpose, proteins were released from the conidial surface by treatme...

journal_title：Archives of microbiology

authors： Messner R,Kubicek-Pranz EM,Gsur A,Kubicek CP

更新日期：1991-01-01 00:00:00

abstract：:A triazine dye, Cibacron blue 3G-A (CB), is an inhibitor of cell separation of staphylococcal spp. therefore, we examined the effect of CB on growth of gram-positive bacteria other than Staphylococcus. CB added to the medium of growing cultures of strains of genus Micrococcus, Streptococcus, Lactobacillus and Bacillus...

journal_title：Archives of microbiology

authors： Sugai M,Akiyama T,Komatsuzawa H,Miyake Y,Suginaka H

更新日期：1991-01-01 00:00:00

abstract：:The isolated cell wall of Streptomyces griseus 52-1 strain labelled with fluorescein isothiocyanate (FITC) and containing wall-bound autolytic enzyme was lysed as a function of different cations. The autolysis was accelerated by aminoglycoside antibiotics (streptomycin and the structurally closely related neomycin) wh...

journal_title：Archives of microbiology

authors： Szabó I,Penyige A,Barabás G,Barabás J

更新日期：1990-01-01 00:00:00

abstract：:Of the seven amino acids required by Clostridium botulinum type E, tryptophan is the most essential and may provide the cell with nitrogen. The addition of excess tryptophan (10-20 mM) or other nitrogenous nutrients to minimal growth medium markedly decreased toxin formation but did not affect growth in C. botulinum t...

journal_title：Archives of microbiology

authors： Leyer GJ,Johnson EA

更新日期：1990-01-01 00:00:00

abstract：:In a soil/sediment model system we have shown recently that a gram-positive bacterium with natural competence (Bacillus subtilis) can take up transforming DNA adsorbed to sand minerals. Here we examined whether also a naturally transformable soil bacterium of the gram-negative pseudomonad (Pseudomonas stutzeri) can be...

journal_title：Archives of microbiology

authors： Lorenz MG,Wackernagel W

更新日期：1990-01-01 00:00:00

abstract：:A 55 kilobase (kb) plasmid (pOZS550) in the non-clumping Lactococcus lactis subsp. lactis strain OZS1 carrying genes for lactose metabolism was characterised. A mobilizable cointegrate plasmid which is formed between pOZS550 and pOZS448 carries the necessary information for conjugation and transfer. Cointegrate format...

journal_title：Archives of microbiology

authors： Smigielski AJ

更新日期：1990-01-01 00:00:00

abstract：:To analyze if chemical cell wall alterations contribute to penicillin-induced bacteriolysis, changes in the amount, stability, and chemical composition of staphylococcal cell walls were investigated. All analyses were performed before onset of bacteriolysis i.e. during the first 60 min following addition of different ...

journal_title：Archives of microbiology

authors： Sidow T,Johannsen L,Labischinski H

更新日期：1990-01-01 00:00:00

abstract：:Listeria monocytogenes was examined for the presence of surface carbohydrates to ascertain whether surface sugars, if present, would interact with eucaryotic surface carbohydrate receptors. We found that a virulent, but not two avirulent strains had a surface alpha-D-galactose residue as determined by agglutination wi...

journal_title：Archives of microbiology

authors： Cowart RE,Lashmet J,McIntosh ME,Adams TJ

更新日期：1990-01-01 00:00:00

abstract：:All Serratia marcescens strains (total of 33) of different sources were hemolytic including clinical strains previously classified as being nonhemolytic. DNA fragments of the two hemolysin genes hybridized with the chromosomal DNA of S. marcescens, S. liquefaciens, S. kiliensis, S. grimesii, S. proteamaculans, S. plym...

journal_title：Archives of microbiology

authors： Ruan Y,Braun V

更新日期：1990-01-01 00:00:00

abstract：:Curcumin is a yellow-orange compound derived from the root of Curcuma longa (Zingiberaceae family), that has been used as a medicine, spice and coloring agent. Curcumin has proved nontoxic in a number of cell culture and whole animal studies. Curcumin has, however, been reported to have bactericidal effects at very hi...

journal_title：Archives of microbiology

authors： Dahl TA,McGowan WM,Shand MA,Srinivasan VS

更新日期：1989-01-01 00:00:00

abstract：:Klebsiella pneumoniae NCTC 418 was cultured aerobically in chemostat cultures (D = 0.3 h-1; 35 degrees C) under respectively carbon-, phosphate-, potassium-, sulphate-, and ammonia-limited conditions with glucose as the sole carbon and energy source. The effect of the external pH value on glucose metabolism and on the...

journal_title：Archives of microbiology

authors： Hommes RW,Postma PW,Tempest DW,Neijssel OM

更新日期：1989-01-01 00:00:00

abstract：:Catalytic properties and membrane associations of the phosphatidylglycerophosphate (PGP) and phosphatidylserine (PS) synthases of Rhodobacter sphaeroides were examined to further characterize sites of phospholipid biosynthesis. In preparations of cytoplasmic membrane (CM) enriched in these activities, apparent Km valu...

journal_title：Archives of microbiology

authors： Radcliffe CW,Steiner FX,Carman GM,Niederman RA

更新日期：1989-01-01 00:00:00

abstract：:The distribution of sex pheromone induced aggregation substance was studied on the cell surface of various Enterococcus faecalis strains. In the accompanying paper we have shown that the aggregation substance appears as a layer of hairlike structures. Using direct and indirect immunogold technique, transmission electr...

journal_title：Archives of microbiology

authors： Wanner G,Formanek H,Galli D,Wirth R

更新日期：1989-01-01 00:00:00

abstract：:Exoglucanase (exo-1,3-beta-D-glucan glycohydrolase, EC 3.2.1.56) activity secreted by Saccharomyces cerevisiae into the culture medium was separated by ion exchange chromatography into two glycoprotein isoenzymes which contributed 10% (exoglucanase I) and 90% (exoglucanase II) towards the total activity. Analysis of t...

journal_title：Archives of microbiology

authors： Ramírez M,Hernández LM,Larriba G

更新日期：1989-01-01 00:00:00

abstract：:The transport of branched-chain amino acids was characterized in intact cells of Corynebacterium glutamicum ATCC 13032. Uptake and accumulation of these amino acids occur via a common specific carrier with slightly different affinities for each substrate (Km[Ile] = 5.4 microM, Km[Leu] = 9.0 microM, Km[Val] = 9.5 micro...

journal_title：Archives of microbiology

authors： Ebbighausen H,Weil B,Krämer R

更新日期：1989-01-01 00:00:00

abstract：:Aquaspirillum magnetotacticum MS-1 cells cultured microaerobically (dissolved O2 tension 1% of saturation), expressed proteins with superoxide dismutase (SOD) activity. The majority (roughly 95%) of total cell superoxide dismutase activity was located in the cell periplasm with little or no activity in the cell cytopl...

journal_title：Archives of microbiology

authors： Short KA,Blakemore RP

更新日期：1989-01-01 00:00:00

abstract：:Diamino butanone (DAB), a competitive inhibitor of ornithine decarboxylase (ODC) a key enzyme in polyamine biosynthesis, inhibited the yeast to hyphae transition in Mucor rouxii, induced by transfer from anaerobiosis to aerobiosis, but not the opposite phenomenon. Addition of DAB to anaerobic yeast cells brought about...

journal_title：Archives of microbiology

authors： Martinez-Pacheco M,Rodriguez G,Reyna G,Calvo-Mendez C,Ruiz-Herrera J

更新日期：1989-01-01 00:00:00

abstract：:The ability of Klebsiella oxytoca NRRL-B199 to use either lactose or the mixture of glucose and galactose as substrate for the production of 2,3-butanediol was studied in batch fermentations with different conditions of aeration and pH. 2,3-butanediol was undetected, or present in minute concentration in the fermentat...

journal_title：Archives of microbiology

authors： Champluvier B,Decallonne J,Rouxhet PG

更新日期：1989-01-01 00:00:00

abstract：:We describe a convenient method for the in vivo construction of large plasmids that possess a multitude of restriction sites. A large (23 kbases) circular self-replicating plasmid carrying a partial LEU2-d gene was cotransformed with a circular non-replicating plasmid carrying the entire LEU2 gene. In vivo recombinati...

journal_title：Archives of microbiology

authors： Valinger R,Braus G,Niederberger P,Künzler M,Paravicini G,Schmidheini T,Hütter R

更新日期：1989-01-01 00:00:00

abstract：:Chloroquine inhibited the outgrowth of Bacillus subtilis spores at a 10-fold lower concentration than that required to prevent vegetative growth. Analysis of macromolecular synthesis in outgrowing spores and vegetative cells in the presence of chloroquine indicated that it acted preferentially on transcription. Differ...

journal_title：Archives of microbiology

authors： Smith KT,Dawes IW

更新日期：1989-01-01 00:00:00

abstract：:The mechanism of lysis of Eubacterium alactolyticum cell walls by Streptomyces albus G enzyme was studied. The analysis of the peptide terminal groups and peptide subunits isolated from the cell wall digest, released during solubilization of the cell walls, revealed that lytic action of S. albus G enzyme was mainly du...

journal_title：Archives of microbiology

authors： Severin AI,Kokeguchi S,Kato K

更新日期：1989-01-01 00:00:00